Cephalosporin C Acylase from Microbes for One-step Enzymatic Transformation of Cephalosporin C to 7-Aminocephalosporanic Acid
نویسندگان
چکیده
منابع مشابه
A single amino acid substitution converts gamma-glutamyltranspeptidase to a class IV cephalosporin acylase (glutaryl-7-aminocephalosporanic acid acylase).
The aspartyl residue at position 433 of gamma-glutamyltranspeptidase of Escherichia coli K-12 was replaced by an asparaginyl residue. This substitution enabled gamma-glutamyltranspeptidase to deacylate glutaryl-7-aminocephalosporanic acid, producing 7-aminocephalosporanic acid, which is a starting material for the synthesis of semisynthetic cephalosporins.
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Several screening methods were developed for the selection of Pseudomonas strains capable of hydrolyzing glutaryl-7-aminocephalosporanic acid to 7-aminocephalosporanic acid. An isolate exhibiting high acylase activity, designated BL072, was identified as a strain of Pseudomonas diminuta. It grew optimally at pH 7 to 8 and at a temperature of 32 to 40 degrees C, but acylase activity was highest ...
متن کاملProcess strategies for cephalosporin C fermentation
Cephalosporin C (CPC), a β-lactam antibiotic, is starting molecule for industrial production of semi synthetic cephalosporins. CPC fermentation has been carried out in Air Lift Reactor (indigenously made up of borosilicate glass) in different fermentation modes i.e., batch and fed batch modes using a mold Acremonium chrysogenum (synn. Cephalosporium acremonium). A definite correlation exists be...
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ژورنال
عنوان ژورنال: Journal of Pure and Applied Microbiology
سال: 2016
ISSN: 0973-7510
DOI: 10.22207/jpam.10.4.03